Difference between revisions of "TC10b Genotyping"
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Latest revision as of 14:55, 5 July 2013
Primers
- TC10b - PDFor1: GGAGTCTATGGACATGAGAG
- TC10b - PD4-43: GATCATGACTAGCATGGAGTAG
- TC10b - PDHomoFB: GTCTATGGACATGAGAGTTTC
- TC10b - NEO3A: GCAGCGCATCGCCTTCTATC
Genotyping Program
- 94 2min
- 50 cycles of:
- 94 30s
- 56 30s
- 72 4min
- 72 7min
- 4 forever
Results
WT allele ~ 1kb, KO allele ~ 500bp HET should get two bands
