Difference between revisions of "Main Page"
From Bridges Lab Protocols
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==Protein Analysis== | ==Protein Analysis== | ||
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*[[Western Blotting]] | *[[Western Blotting]] | ||
*[[Surface Plasmon Resonance - Protein Lipid Interactions]] | *[[Surface Plasmon Resonance - Protein Lipid Interactions]] | ||
| + | ===Protein Quantification=== | ||
| + | *[[Bradford Assay]] | ||
| + | *[[Quantification by Absorbance at 280nm]] | ||
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===Protein Purification=== | ===Protein Purification=== | ||
*[[French Press]] | *[[French Press]] | ||
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*[[DNA Preparation from Tail Clip]] | *[[DNA Preparation from Tail Clip]] | ||
*[[PCR Analysis of Tail DNA]] | *[[PCR Analysis of Tail DNA]] | ||
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Revision as of 13:43, 11 May 2009
Contents
Cell and Tissue Culture
- Splitting Cells
- Differentiation of 3T3-L1 Cells
- Electroporation of 3T3-L1 Adipocytes
- Immunofluoresence
- 3T3-L1 Adipocyte Fractionation
Cloning and Molecular Biology
- Transformation of Bacteria
- Mutagenesis
- PCR Amplification of DNA
- Restriction Enzyme Based Cloning
- TOPO Cloning
- Preparing an Agarose Gel
