PCR Analysis of Tail DNA

see Genotyping Details for strain specific details

Materials

1. Dream Taq Green master mix
2. Specific gene Primers (0.4um Working stock)
3. Tail digest DNA
4. ddH2O

Protocol

Use the following Volumes per 25ul Reaction:

Per sample 1X

1. Dream Tag Master mix: 12.5uL ("Molecular Biology Stuff" box in freezer)
2. Primer Mix: 5ul
3. Sterile ddH2O: 7.5ul

• Template: 1 uL

Run "specfic" PCR Program for gene of interest (approx 2 hours).

see Preparing an Agarose Gel for details on preparing a DNA gel