PCR Analysis of Tail DNA
From Bridges Lab Protocols
see Genotyping Details for strain specific details
Materials
Protocol
Use the following Volumes per Reaction:
Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer)
Forward Primer: .4ul
Reverse Primer: .4ul
dNTPs: .4uL of 2 mM ("Molecular Biology Stuff" box in freezer)
Sterile water: 13.6 uL
Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer)
Template: 1 uL
see Preparing an Agarose Gel for details on preparing a DNA gel
