GoTaq PCR Genotyping

Materials

• GoTaq Master Mix
• Primer Mix (100uL Primers): Add 10uL of forward and reverse primer, 20uL total, into 1mL of dH20.
• Reaction Mixture: 25uL GoTaq Mix, 10uL of Primer Mix, 14uL Nuclease-Free Water, and 1uL of template
• Gel: 0.3g Agarose to 30mL of TAE, microwave until dissolved and add 1uL of EtBr.

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Procedure

• Prepare gel 30 minutes before PCR is finished.
• Prepare Reaction Mixture, adding 13x all materials except for the template.
• Add this mixture to each PCR tube.
• Label and add each template to the corresponding PCR tube.
• Run PCR under genotyping>inoki (~1 hour)
• Load samples in gel and run on 30V (horizontally.)