Designing siRNA for Knockdown
From Bridges Lab Protocols
- Use Invitrogen's BLOCK-IT RNAi Designer [1]
- Find the refseq RNA sequence for your gene by going to Entrez Gene and entering the gene name and sequence. The RefSeq sequence should start with "NM_"
- Select the appropriate control species and whether to target the coding sequence (normally what we do), or the UTRs
- You will usually get a list of several potential sequences, ordered by location on the RNA molecule. Pick 2-3 sequences, based on the alternative splicing of the RNA. If there is only one listed splice variant on NCBI Gene, pick one towards the N-terminus and one towards the C-terminus.
- Select continue
- Click the "tube" button and disregard the scrambled RNA controls.
- Click "Order Online" button.
- Add your name to Reseacher Name and order.
- Use Promo code P756389 until 30 Sept 2011
