Designing and Preparing dsRNA

From Bridges Lab Protocols
Revision as of 13:27, 3 August 2009 by Davebridges (Talk | contribs) (intial page)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

Ordering dsRNA Primers

  • If available choose primers from Harvard DSRC site http://www.flyrnai.org/
  • Add T7 RNA Polymerase sites to the 5' of each primer:

TAATACGACTCACTATAGG