Designing and Preparing dsRNA
From Bridges Lab Protocols
Revision as of 13:47, 3 August 2009 by Davebridges (Talk | contribs)
Ordering dsRNA Primers
- If available, choose primers from Harvard DSRC site http://www.flyrnai.org/cgi-bin/RNAi_gene_lookup_public.pl
- Add T7 RNA Polymerase sites to the 5' of each primer (TAATACGACTCACTATAGG)
- Record primer and amplicon (DSRC)
