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- 01:06, 2 May 2009 (diff | hist) . . (+41) . . Differentiation of 3T3-L1 Cells
- 01:00, 2 May 2009 (diff | hist) . . (+92) . . N Bridges Lab Protocols:About (Created page with 'This page contains protocols developed by and for use in the laboratory of Dr. Alan Saltiel.')
- 00:58, 2 May 2009 (diff | hist) . . (+58) . . Main Page
- 00:58, 2 May 2009 (diff | hist) . . (+1,476) . . N Restriction Enzyme Based Cloning - Ordering Primers (Created page with '==Preparation and Ordering Primers== #Choose target vector and region of interest in gene #Pick 5' and 3' restriction sites that are present in the vector but NOT in the insert #...')
- 00:54, 2 May 2009 (diff | hist) . . (+32) . . Western Blotting (→Protocol)
- 00:53, 2 May 2009 (diff | hist) . . (+4) . . Western Blotting (→Materials)
- 00:51, 2 May 2009 (diff | hist) . . (+22) . . Main Page
- 00:51, 2 May 2009 (diff | hist) . . (+1,232) . . N Western Blotting (Created page with '==Materials== *Transfer Buffer (100 mL Methanol, 50 mL 10X Transfer Buffer to final 500 mL) *Transfer Apparatus, either Bio-Rad or Invitrogen ==Protocol== #Run SDS-PAGE gel and ...')
- 00:45, 2 May 2009 (diff | hist) . . (+18) . . Main Page (→Cloning and Molecular Biology)
- 00:45, 2 May 2009 (diff | hist) . . (+611) . . N TOPO Cloning (Created page with '#PCR desired insert using Pfu polymerase. Add 1 uL of Taq and mix. Incubate 10min at 72C to add A to 3’ ends. Use immediately #If PCR product is a single band, no need to pu...') (current)
- 00:40, 2 May 2009 (diff | hist) . . (+60) . . Main Page
- 00:39, 2 May 2009 (diff | hist) . . (+41) . . Surface Plasmon Resonance - Protein Lipid Interactions
- 00:35, 2 May 2009 (diff | hist) . . (+1,640) . . N Surface Plasmon Resonance - Protein Lipid Interactions (Created page with '==Materials== *L1 Sensor Chip (Biacore) *Dioleylphosphatidylcholine (Sigma) dissolved in 1:1 chloroform:methanol with 0.1% HCl *PIPx of interest (Avanti or Echelon) dissolved in ...')
- 00:31, 2 May 2009 (diff | hist) . . (+55) . . Main Page (→Cloning and Molecular Biology)
- 00:31, 2 May 2009 (diff | hist) . . (+1,020) . . N Restriction Enzyme Based Cloning (copied over protocol)
- 00:29, 2 May 2009 (diff | hist) . . (+13) . . Mutagenesis (→Protocol)
- 00:29, 2 May 2009 (diff | hist) . . (0) . . Mutagenesis (→Protocol)
- 00:28, 2 May 2009 (diff | hist) . . (+1,049) . . N Mutagenesis (copied over protocol)
- 00:08, 2 May 2009 (diff | hist) . . (+23) . . Main Page (→Cell and Tissue Culture)
- 00:08, 2 May 2009 (diff | hist) . . (+1) . . Immunofluoresence
- 00:07, 2 May 2009 (diff | hist) . . (+1,242) . . N Immunofluoresence (copied over protocol)
- 23:58, 1 May 2009 (diff | hist) . . (+2) . . m Main Page (→Transcriptional Analysis)
- 23:57, 1 May 2009 (diff | hist) . . (-273) . . Main Page (→Getting started: removed section)
- 22:53, 1 May 2009 (diff | hist) . . (+621) . . N Bradford Assay (copied over protocol)
- 22:50, 1 May 2009 (diff | hist) . . (+109) . . Main Page
- 22:48, 1 May 2009 (diff | hist) . . (+567) . . N Transformation of Bacteria (copied over protocol)
- 22:26, 1 May 2009 (diff | hist) . . (+2,942) . . N Differentiation of 3T3-L1 Cells (copied over page)
- 22:23, 1 May 2009 (diff | hist) . . (+81) . . Main Page (→Protocols: added links)
- 20:02, 1 May 2009 (diff | hist) . . (+26) . . Real Time PCR From Cell Culture (→References (Saltiel Lab): added Chang et al Mol Endocrinol)
- 20:00, 1 May 2009 (diff | hist) . . (+31) . . N Help:Contents (wrote initial help page)
- 19:51, 1 May 2009 (diff | hist) . . (+78) . . Real Time PCR From Cell Culture (→References (Saltiel Lab): added references)
- 19:45, 1 May 2009 (diff | hist) . . (-62) . . m Main Page
- 19:44, 1 May 2009 (diff | hist) . . (+53) . . Main Page
- 19:41, 1 May 2009 (diff | hist) . . (+31) . . Real Time PCR From Cell Culture
- 19:39, 1 May 2009 (diff | hist) . . (+7,157) . . N Help:Editing (Created page with 'from http://meta.wikimedia.org/wiki/Help:Editing#Wiki_markups_and_codes {{H:h|editor toc}} '''Editing''' refers to the changing of a page by a Wikimedia user. Unless certain re...') (current)
- 19:37, 1 May 2009 (diff | hist) . . (+1,129) . . N Real Time PCR From Cell Culture (Created page with 'Real Time qPCR Materials cDNA for templates Qiashredder and RNEasy kits from Qiagen Superscript Kit from Invitrogen SyberGreen PCR Master Mix Applied Biosystems 96 well qPCR plat...')
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