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- 17:29, 7 May 2009 (diff | hist) . . (+42) . . Main Page (→Cell and Tissue Culture)
- 14:40, 7 May 2009 (diff | hist) . . (+1) . . m Restriction Enzyme Based Cloning (current)
- 14:22, 7 May 2009 (diff | hist) . . (+252) . . Restriction Enzyme Based Cloning (updated with gel purification)
- 18:52, 6 May 2009 (diff | hist) . . (+2) . . PCR Analysis of Tail DNA
- 18:52, 6 May 2009 (diff | hist) . . (+2) . . PCR Analysis of Tail DNA (→Protocol)
- 18:52, 6 May 2009 (diff | hist) . . (+148) . . N PCR Analysis of Tail DNA (Created page with 'see [Genotyping Details] for strain specific details ==Materials== ==Protocol== see [Preparing an Agarose Gel] for details on preparing a DNA gel')
- 18:50, 6 May 2009 (diff | hist) . . (+139) . . Main Page
- 15:16, 6 May 2009 (diff | hist) . . (+12) . . m Induction Conditions
- 15:15, 6 May 2009 (diff | hist) . . (-54) . . m Induction Conditions
- 15:15, 6 May 2009 (diff | hist) . . (-9) . . m Induction Conditions
- 15:14, 6 May 2009 (diff | hist) . . (+218) . . N Induction Conditions (Created page with '{| class="wikitable" |- ! Protein ! IPTG Concentration ! Time ! Temperature ! Notes |- | some protein | some IPTG | some time | some temperature | some note |- | row 2, cel...')
- 15:12, 6 May 2009 (diff | hist) . . (+30) . . m Purification of GST Fusion Proteins
- 15:09, 6 May 2009 (diff | hist) . . (+21) . . Main Page (→Cell and Tissue Culture)
- 15:08, 6 May 2009 (diff | hist) . . (+519) . . N Splitting Cells (Created page with '==Materials== *FBS (L1-FBS for 3T3-L1, COS-FBS for others): Prepare by filtering together 5 mL PSG, 50 mL COS-FBS and one bottle of DMEM *PBS -/- *0.05% Trypsin ==Protocol== #W...')
- 20:19, 5 May 2009 (diff | hist) . . (+1) . . m Restriction Enzyme Based Cloning - Ordering Primers (→Preparation and Ordering Primers) (current)
- 16:03, 5 May 2009 (diff | hist) . . (+484) . . N Preparing an Agarose Gel (wrote page)
- 16:00, 5 May 2009 (diff | hist) . . (+30) . . Main Page
- 15:08, 5 May 2009 (diff | hist) . . (+30) . . Main Page
- 15:08, 5 May 2009 (diff | hist) . . (+41) . . Main Page
- 15:07, 5 May 2009 (diff | hist) . . (+1,767) . . N Purification of GST Fusion Proteins (Created page with '==Bacteria Production and Induction== #Express and induce protein in culture under appropriate conditions (normally do the following) #grow an overnight culture in ~25 mL LB/Amp ...')
- 13:25, 5 May 2009 (diff | hist) . . (0) . . m PCR Amplification of DNA (→Protocol)
- 13:24, 5 May 2009 (diff | hist) . . (+11) . . m PCR Amplification of DNA (→Protocol)
- 13:09, 5 May 2009 (diff | hist) . . (-11) . . m PCR Amplification of DNA
- 13:09, 5 May 2009 (diff | hist) . . (+1) . . PCR Amplification of DNA
- 13:08, 5 May 2009 (diff | hist) . . (+11) . . m PCR Amplification of DNA
- 12:53, 5 May 2009 (diff | hist) . . (+941) . . N PCR Amplification of DNA (Created page with '==Materials== *Primers – order from IDT prediluted to 100 mM in TE. Make working solution of 1 uM (100x) in water with both sense and antisense primers combined *dNTPs – dil...')
- 12:40, 4 May 2009 (diff | hist) . . (-8) . . 3T3-L1 Adipocyte Fractionation (→Optiprep Gradient)
- 12:39, 4 May 2009 (diff | hist) . . (+36) . . Main Page (→Cell and Tissue Culture)
- 12:38, 4 May 2009 (diff | hist) . . (+156) . . 3T3-L1 Adipocyte Fractionation
- 12:35, 4 May 2009 (diff | hist) . . (+1,336) . . N 3T3-L1 Adipocyte Fractionation (Created page with '==Materials== *PBS *Lysis Buffer (25 mM HEPES, 250 mM Sucrose, PI tablet) *Optiprep ==Fractionation== #Wash cells twice with ice cold PBS -/- #Scrape 150 mm dish into 4 mL of Ly...')
- 12:22, 4 May 2009 (diff | hist) . . (+1,289) . . N French Press (imported protocol)
- 12:20, 4 May 2009 (diff | hist) . . (+45) . . Main Page
- 01:31, 4 May 2009 (diff | hist) . . (-2) . . Help:Contents (current)
- 01:30, 4 May 2009 (diff | hist) . . (-2) . . Help:Contents
- 01:30, 4 May 2009 (diff | hist) . . (0) . . Help:Contents
- 01:28, 4 May 2009 (diff | hist) . . (+20) . . Help:Contents
- 01:26, 4 May 2009 (diff | hist) . . (+12) . . Help:Contents (→Editing)
- 01:26, 4 May 2009 (diff | hist) . . (+42) . . Help:Contents
- 01:25, 4 May 2009 (diff | hist) . . (+20,399) . . N Help:Preventing access (Created page with '{{languages}} For help customizing user rights, see Manual:User rights. This page contains examples useful for restricting access. Most of the examples need changes to [[Ma...') (current)
- 01:03, 4 May 2009 (diff | hist) . . (+2) . . Bradford Assay (→Reference)
- 01:03, 4 May 2009 (diff | hist) . . (+69) . . Bradford Assay
- 01:00, 4 May 2009 (diff | hist) . . (+26) . . Help:Contents
- 00:59, 4 May 2009 (diff | hist) . . (+21) . . Help:Contents (→Editing)
- 00:57, 4 May 2009 (diff | hist) . . (-11) . . Help:Wikitext examples (current)
- 00:56, 4 May 2009 (diff | hist) . . (+28) . . Help:Contents
- 00:56, 4 May 2009 (diff | hist) . . (+19,408) . . N Help:Wikitext examples (Created page with 'from http://en.wikipedia.org/w/index.php?title=Help:Wikitext_examples For basic information see Help:Editing. == Basic text formatting == You can format the page using Wikit...')
- 00:52, 4 May 2009 (diff | hist) . . (-2) . . m Restriction Enzyme Based Cloning - Ordering Primers
- 00:52, 4 May 2009 (diff | hist) . . (-3) . . m Restriction Enzyme Based Cloning - Ordering Primers
- 00:51, 4 May 2009 (diff | hist) . . (0) . . m Restriction Enzyme Based Cloning - Ordering Primers
- 00:49, 4 May 2009 (diff | hist) . . (+76) . . Restriction Enzyme Based Cloning - Ordering Primers (added ncbi primer blast)
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