User contributions
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- 17:59, 5 June 2009 (diff | hist) . . (-4) . . m PCR Analysis of Tail DNA
- 17:58, 5 June 2009 (diff | hist) . . (-23) . . m DNA Preparation from Tail Clip (current)
- 23:55, 3 June 2009 (diff | hist) . . (+61) . . Immunofluoresence (→Protocol: added link)
- 20:02, 2 June 2009 (diff | hist) . . (0) . . Luciferase Assay
- 20:02, 2 June 2009 (diff | hist) . . (+1,256) . . N Luciferase Assay (initial page)
- 20:01, 2 June 2009 (diff | hist) . . (+22) . . Main Page (→Cell and Tissue Culture)
- 17:18, 2 June 2009 (diff | hist) . . (+39) . . Immunofluoresence (→Protocol)
- 12:13, 28 May 2009 (diff | hist) . . (+123) . . QPCR - Lin Lab (→Before the run:: modified machine setup) (current)
- 12:09, 28 May 2009 (diff | hist) . . (-2) . . m Real Time PCR From Cell Culture (→Plate Preparation)
- 22:02, 27 May 2009 (diff | hist) . . (-33) . . m QPCR - Lin Lab (→PCR Program:)
- 21:58, 27 May 2009 (diff | hist) . . (+42) . . m QPCR - Lin Lab (→PCR Program:)
- 21:55, 27 May 2009 (diff | hist) . . (+13) . . m QPCR - Lin Lab (→PCR Program:)
- 21:55, 27 May 2009 (diff | hist) . . (+79) . . m QPCR - Lin Lab (→After the run:)
- 21:51, 27 May 2009 (diff | hist) . . (+3,095) . . Nm QPCR - Lin Lab (Created page with '==Before the run:== #Click on “Shortcut to Sybr plate” icon. #Click on “Instrument” tab and change volume of rxn. to 20uL. With new primers, may want to run the dissociat...')
- 21:48, 27 May 2009 (diff | hist) . . (+122) . . Real Time PCR From Cell Culture (→Plate Preparation)
- 14:24, 27 May 2009 (diff | hist) . . (+1,032) . . N Cesium Chloride Preparation of DNA (wrote initial page)
- 14:22, 27 May 2009 (diff | hist) . . (+40) . . m Main Page (→Cloning and Molecular Biology)
- 13:24, 27 May 2009 (diff | hist) . . (+427) . . N Check Clones for Correct Mutation (wrote initial page) (current)
- 13:06, 27 May 2009 (diff | hist) . . (+40) . . Mutagenesis (→Protocol: added link to checking clones)
- 00:32, 27 May 2009 (diff | hist) . . (+16) . . m Preparing Cell Lysates (→Basic Protocol)
- 00:29, 27 May 2009 (diff | hist) . . (+424) . . N Buffer/RIPA (initial page)
- 00:15, 27 May 2009 (diff | hist) . . (0) . . Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B) (→Materials) (current)
- 00:15, 27 May 2009 (diff | hist) . . (-221) . . m Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)
- 00:13, 27 May 2009 (diff | hist) . . (+17) . . Glucose Uptake Assay
- 21:37, 26 May 2009 (diff | hist) . . (-50) . . Main Page
- 21:37, 26 May 2009 (diff | hist) . . (+816) . . N Glucose Uptake Assay (initial page)
- 21:29, 26 May 2009 (diff | hist) . . (+26) . . m Main Page
- 21:25, 26 May 2009 (diff | hist) . . (+123) . . Preparing Cell Lysates (edited phosphatase inhibitors)
- 21:15, 26 May 2009 (diff | hist) . . (+2) . . m Preparing Cell Lysates (→RIPA Buffer (for 10mL lysis buffer))
- 21:14, 26 May 2009 (diff | hist) . . (+117) . . Preparing Cell Lysates (edited table)
- 20:12, 26 May 2009 (diff | hist) . . (-28) . . Real Time PCR From Cell Culture (→Plate Preparation)
- 18:51, 26 May 2009 (diff | hist) . . (+82) . . Real Time PCR From Cell Culture (→RT-PCR Reaction)
- 15:07, 20 May 2009 (diff | hist) . . (+79) . . Glucose Tolerance Test
- 15:42, 13 May 2009 (diff | hist) . . (+116) . . Glucose Tolerance Test (→Protocol)
- 15:41, 13 May 2009 (diff | hist) . . (+21) . . Glucose Tolerance Test (→Materials (Bring Downstairs))
- 13:43, 13 May 2009 (diff | hist) . . (+2) . . Main Page (→Metabolic Measurements)
- 13:43, 13 May 2009 (diff | hist) . . (+847) . . N Glucose Tolerance Test (wrote initial page)
- 13:32, 13 May 2009 (diff | hist) . . (+55) . . Main Page (→Genotyping)
- 13:31, 13 May 2009 (diff | hist) . . (+50) . . Main Page (→Transcriptional Analysis)
- 15:22, 11 May 2009 (diff | hist) . . (-5) . . Main Page (→Cell and Tissue Culture)
- 15:22, 11 May 2009 (diff | hist) . . (+33) . . Main Page (→Cell and Tissue Culture)
- 15:21, 11 May 2009 (diff | hist) . . (+19) . . N Preparing Cell Lysates (Created page with 'type something here')
- 14:10, 11 May 2009 (diff | hist) . . (+50) . . N DNA Preparation from Tail Clip (Created page with 'Use Qiagen DNEasy kit for Blood and Tissue Samples')
- 14:09, 11 May 2009 (diff | hist) . . (+32) . . Main Page (→Protein Purification)
- 14:09, 11 May 2009 (diff | hist) . . (+611) . . N Determining Percent Purity (wrote initial page)
- 14:03, 11 May 2009 (diff | hist) . . (0) . . Quantification by Absorbance at 280nm (current)
- 14:02, 11 May 2009 (diff | hist) . . (+702) . . N Quantification by Absorbance at 280nm (wrote initial page)
- 13:43, 11 May 2009 (diff | hist) . . (-46) . . Main Page
- 17:41, 7 May 2009 (diff | hist) . . (+17) . . Electroporation of 3T3-L1 Adipocytes
- 17:39, 7 May 2009 (diff | hist) . . (+858) . . N Electroporation of 3T3-L1 Adipocytes (Created page with '==Materials== *Differentiated cells FBS day 3 or less. *Gene Pulser cuvette *DNA: CsCl Purified or siRNA (resuspended to 0.1 nmol/uL with 200 uL water) *PBS +/+ *PBS -/- *0.25% ...')
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