User contributions
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- 16:51, 20 August 2009 (diff | hist) . . (+10) . . m Real Time PCR From Cell Culture
- 15:48, 13 August 2009 (diff | hist) . . (+1) . . m Generating DMSO Stocks for Cell Culture
- 15:44, 13 August 2009 (diff | hist) . . (+50) . . m Generating DMSO Stocks for Cell Culture
- 15:42, 13 August 2009 (diff | hist) . . (+45) . . m Main Page (→Cell and Tissue Culture)
- 15:41, 13 August 2009 (diff | hist) . . (+302) . . m Generating DMSO Stocks for Cell Culture
- 15:35, 13 August 2009 (diff | hist) . . (+577) . . N Generating DMSO Stocks for Cell Culture (Created page with '==Materials== *Cells in 10cm dishes, at 90-95% confluence. *Cryopreservation Container (Nalgene 5100-0001) *Sterile DMSO ==Protocol== #Pick a low passage number of cells and gro...')
- 14:45, 13 August 2009 (diff | hist) . . (+28) . . m Lipofectamine Plasmid Transfection (→Protocol (6 well dish))
- 18:18, 12 August 2009 (diff | hist) . . (-61) . . m Lipofectamine Plasmid Transfection (→Protocol (6 well dish))
- 17:57, 12 August 2009 (diff | hist) . . (+594) . . m Lipofectamine Plasmid Transfection
- 17:50, 12 August 2009 (diff | hist) . . (+295) . . N Lipofectamine Plasmid Transfection (intial page)
- 17:48, 12 August 2009 (diff | hist) . . (0) . . m Lipofectamine Mediated Knockdown
- 17:48, 12 August 2009 (diff | hist) . . (+10) . . m Lipofectamine Mediated Knockdown
- 17:48, 12 August 2009 (diff | hist) . . (+27) . . m Lipofectamine Mediated Knockdown
- 17:48, 12 August 2009 (diff | hist) . . (+40) . . m Main Page (→Cell and Tissue Culture)
- 14:40, 12 August 2009 (diff | hist) . . (+10) . . m Purification of GST Fusion Proteins (current)
- 14:39, 12 August 2009 (diff | hist) . . (+46) . . m Purification of GST Fusion Proteins
- 14:39, 12 August 2009 (diff | hist) . . (+41) . . m Purification of GST Fusion Proteins
- 14:37, 12 August 2009 (diff | hist) . . (+34) . . m Purification of GST Fusion Proteins
- 14:37, 12 August 2009 (diff | hist) . . (+34) . . m Induction Conditions
- 14:36, 12 August 2009 (diff | hist) . . (+163) . . Induction Conditions (added hrs and fyve)
- 14:34, 12 August 2009 (diff | hist) . . (+56) . . Purification of GST Fusion Proteins (→Bacteria Production and Induction)
- 15:44, 7 August 2009 (diff | hist) . . (+100) . . m Surface Plasmon Resonance - Protein Lipid Interactions
- 15:44, 7 August 2009 (diff | hist) . . (+86) . . m Inositol Labeling and Lipid Extraction (current)
- 15:43, 7 August 2009 (diff | hist) . . (+44) . . m Main Page (→Cell and Tissue Culture)
- 15:42, 7 August 2009 (diff | hist) . . (-155) . . m Inositol Labeling and Lipid Extraction (→References)
- 15:41, 7 August 2009 (diff | hist) . . (-21) . . m Inositol Labeling and Lipid Extraction (→Materials)
- 15:40, 7 August 2009 (diff | hist) . . (+1,682) . . N Inositol Labeling and Lipid Extraction (Created page with '==Materials== �*Cells in 100 mm culture dishes �*Labelling Media (Inositol Free DMEM with PSG, 10% dialysed serum and 100 uCi inositol per plate of cells) �*Perchloric acid...')
- 13:12, 7 August 2009 (diff | hist) . . (-12) . . Surface Plasmon Resonance - Protein Lipid Interactions
- 13:11, 7 August 2009 (diff | hist) . . (+10) . . Surface Plasmon Resonance - Protein Lipid Interactions
- 12:08, 5 August 2009 (diff | hist) . . (+102) . . m Preparing a SDS-PAGE Gel
- 12:07, 5 August 2009 (diff | hist) . . (-8) . . m Preparing a SDS-PAGE Gel
- 12:06, 5 August 2009 (diff | hist) . . (+113) . . Preparing a SDS-PAGE Gel (added 6% gel)
- 18:58, 3 August 2009 (diff | hist) . . (+30) . . m Main Page (→Protein Analysis)
- 18:57, 3 August 2009 (diff | hist) . . (-45) . . Acrylamide Solution (current)
- 18:56, 3 August 2009 (diff | hist) . . (+182) . . N SDS-PAGE Stacking Gel Buffer (Created page with 'Category:SDS-PAGE Category:Electrophoresis Category:Buffer For 1L: *60.56g Tris *40 mL 10% SDS (or 4 g SDS) *Add water to near 1L *Adjust pH to 6.8 *Bring volume up t...') (current)
- 18:56, 3 August 2009 (diff | hist) . . (+183) . . N SDS-PAGE Separating Gel Buffer (Created page with 'Category:SDS-PAGE Category:Electrophoresis Category:Buffer For 1L: *181.66g Tris *40 mL 10% SDS (or 4 g SDS) *Add water to near 1L *Adjust pH to 8.8 *Bring volume up ...') (current)
- 18:55, 3 August 2009 (diff | hist) . . (-1) . . Acrylamide Solution
- 18:55, 3 August 2009 (diff | hist) . . (+184) . . N Acrylamide Solution (initial page)
- 18:52, 3 August 2009 (diff | hist) . . (+939) . . N Preparing a SDS-PAGE Gel (Created page with '==Separating Gel== *For 12 mL final volume (2 gels) *Combine, adding APS and TEMED last *Pour into cassette, overlay with the top layer of water saturated butanol {| border="1" !...')
- 13:47, 3 August 2009 (diff | hist) . . (+63) . . m Designing and Preparing dsRNA
- 13:44, 3 August 2009 (diff | hist) . . (+71) . . m Designing and Preparing dsRNA
- 13:27, 3 August 2009 (diff | hist) . . (+183) . . N Designing and Preparing dsRNA (intial page)
- 13:23, 3 August 2009 (diff | hist) . . (+92) . . m Culturing S2 Cells (current)
- 16:07, 31 July 2009 (diff | hist) . . (+28) . . m Freezing and Thawing S2 Cells
- 16:07, 31 July 2009 (diff | hist) . . (+553) . . N Freezing and Thawing S2 Cells (initial page)
- 16:03, 31 July 2009 (diff | hist) . . (-47) . . m Culturing S2 Cells (→Materials)
- 16:00, 31 July 2009 (diff | hist) . . (+42) . . m Culturing S2 Cells
- 15:43, 31 July 2009 (diff | hist) . . (+516) . . m Culturing S2 Cells
- 15:37, 31 July 2009 (diff | hist) . . (+221) . . m Culturing S2 Cells
- 15:35, 31 July 2009 (diff | hist) . . (+76) . . m Culturing S2 Cells
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