User contributions
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- 14:43, 16 June 2010 (diff | hist) . . (+2,013) . . N Immobilizing a Protein on a CM5 Chip for SPR (wrote initial page) (current)
- 13:42, 11 June 2010 (diff | hist) . . (+752) . . N Quantification of Vacuolar Colocalization (wrote initial page) (current)
- 20:16, 24 May 2010 (diff | hist) . . (+1,466) . . N TAP Purification of Yeast Extracts (wrote initial protocol from Seraphin lab page) (current)
- 19:23, 14 May 2010 (diff | hist) . . (+10) . . m HPLC - Plumbing Setup (removed TOC) (current)
- 19:22, 14 May 2010 (diff | hist) . . (+2,357) . . HPLC - Designing a Method for a Sample or Series (filled in details for method design) (current)
- 18:39, 14 May 2010 (diff | hist) . . (+48) . . HPLC - Designing a Method for a Sample or Series (left space for notes)
- 18:38, 14 May 2010 (diff | hist) . . (+11) . . HPLC - Running a Sample or Series (removed TOC) (current)
- 18:38, 14 May 2010 (diff | hist) . . (+2,629) . . N HPLC - Running a Sample or Series (wrote initial instructions)
- 17:20, 14 May 2010 (diff | hist) . . (+610) . . N HPLC - Designing a Method for a Sample or Series (wrote part of initial page)
- 16:29, 14 May 2010 (diff | hist) . . (+4,096) . . N HPLC - Plumbing Setup (wrote initial instructions)
- 15:34, 11 May 2010 (diff | hist) . . (+2,751) . . Surface Plasmon Resonance - Protein Lipid Interactions (Updated protocol with more detailed notes.)
- 16:57, 6 May 2010 (diff | hist) . . (+376) . . Glycogen Determination from Tissues (added glucose quantification protocol)
- 15:01, 6 May 2010 (diff | hist) . . (+733) . . N Glycogen Determination from Tissues (Wrote initial protocol)
- 14:05, 28 April 2010 (diff | hist) . . (+122) . . 3T3-L1 Adipocyte Fractionation (modified volumes to reflect whether using optiprep or not.)
- 15:24, 27 April 2010 (diff | hist) . . (+130) . . Glycogen Synthase Assay (clarified some points)
- 16:45, 26 April 2010 (diff | hist) . . (+813) . . N Using Git (wrote initial page) (current)
- 13:42, 23 April 2010 (diff | hist) . . (-10) . . m Bradford Assay (formatting change for PMID)
- 20:11, 30 March 2010 (diff | hist) . . (+76) . . Main Page (→Protein Analysis: added links to Glycogen Synthase and Triglyceride Assays)
- 20:11, 30 March 2010 (diff | hist) . . (+847) . . N Triglyceride Assay from Cells and Tissues (wrote initial page)
- 19:49, 30 March 2010 (diff | hist) . . (+1,289) . . N Glycogen Synthase Assay (wrote initial page)
- 16:14, 8 March 2010 (diff | hist) . . (+24) . . Cesium Chloride Preparation of DNA (added concentration to Acetate Buffer)
- 16:08, 24 February 2010 (diff | hist) . . (+74) . . Glucose Incorporation into Lipid and Glycogen (added details about protein assay)
- 16:07, 24 February 2010 (diff | hist) . . (+2,047) . . Glucose Incorporation into Lipid and Glycogen (filled in details from Brady protocol.)
- 18:45, 17 February 2010 (diff | hist) . . (+525) . . Electroporation of 3T3-L1 Adipocytes (changed default siRNA to 5 uL (500 pmoles) and made some extra notes) (current)
- 21:01, 8 February 2010 (diff | hist) . . (+27) . . N Glucose Incorporation into Lipid and Glycogen (initial skeleton)
- 17:04, 2 February 2010 (diff | hist) . . (+11) . . m Yeast Sch9 Phosphorylation Assay
- 16:53, 2 February 2010 (diff | hist) . . (-1,595) . . Yeast Sch9 Phosphorylation Assay (modified to a protocol)
- 15:24, 27 January 2010 (diff | hist) . . (-3) . . FM4-64 Labeling of Yeast Cells (updated synaptored concentration) (current)
- 16:26, 20 January 2010 (diff | hist) . . (+69) . . FM4-64 Labeling of Yeast Cells (Changed dye concentration to match klionsky protocol)
- 16:22, 15 January 2010 (diff | hist) . . (-581) . . Yeast Sch9 Phosphorylation Assay
- 16:17, 15 January 2010 (diff | hist) . . (-2) . . Yeast Sch9 Phosphorylation Assay
- 16:16, 15 January 2010 (diff | hist) . . (+3,244) . . N Yeast Sch9 Phosphorylation Assay (Created page with 'from PMID 17560372 Kinase Assay TORC1 was purified from RL175-2d or RL176-1b cells treated with drug vehicle or 200 nM rapamycin for 30 min. Cells grown at 30°C in YPD (250 ...')
- 15:15, 13 January 2010 (diff | hist) . . (+178) . . Mutagenesis (updated for new PCR program and to use Pfu Turbo as the polymerase) (current)
- 17:14, 22 December 2009 (diff | hist) . . (+1) . . m Yeast Transformation (typo with dna volume) (current)
- 16:48, 22 December 2009 (diff | hist) . . (0) . . m Yeast Transformation (table formatting error)
- 15:49, 22 December 2009 (diff | hist) . . (+24) . . m Connect to Network Drives (current)
- 15:47, 22 December 2009 (diff | hist) . . (0) . . m Connect to Network Drives
- 15:47, 22 December 2009 (diff | hist) . . (+543) . . N Connect to Network Drives (wrote initial page)
- 17:56, 21 December 2009 (diff | hist) . . (+1) . . m Yeast Transformation
- 17:56, 21 December 2009 (diff | hist) . . (+63) . . Yeast Transformation (added link to agar plates)
- 17:54, 21 December 2009 (diff | hist) . . (+1,913) . . N Yeast Transformation (wrote initial page)
- 15:28, 21 December 2009 (diff | hist) . . (+70) . . RIPA Buffer (changed vanadate concentration and volume)
- 16:39, 18 December 2009 (diff | hist) . . (0) . . m Lipofectamine Mediated Knockdown (fixed typo pmoles should be nmoles) (current)
- 16:38, 18 December 2009 (diff | hist) . . (-2,706) . . Lipofectamine Mediated Knockdown (rewrote according to lipofectamine instructions)
- 14:10, 15 December 2009 (diff | hist) . . (+12) . . m Main Page (added bullet points)
- 14:09, 15 December 2009 (diff | hist) . . (+417) . . Main Page (added media and buffers to index page)
- 14:02, 15 December 2009 (diff | hist) . . (+1,248) . . N Yeast Selective Media and Agar (wrote initial page)
- 15:02, 14 December 2009 (diff | hist) . . (-54) . . m Yeast Transfection (Small Scale) (formatting changes) (current)
- 15:01, 14 December 2009 (diff | hist) . . (+1,688) . . N Yeast Transfection (Small Scale) (initial copy from protocol folder)
- 19:51, 7 December 2009 (diff | hist) . . (+54) . . m Main Page
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