User contributions
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- 21:16, 11 November 2011 (diff | hist) . . (-1) . . m Glucose Incorporation into Lipid and Glycogen (changed template link)
- 21:15, 11 November 2011 (diff | hist) . . (+390) . . Glucose Incorporation into Lipid and Glycogen (added some extra notes and a link to the template)
- 16:08, 8 November 2011 (diff | hist) . . (+207) . . RNA-seq Pipeline for Known Transcripts (added section for aligning reads with bowtie) (current)
- 16:02, 8 November 2011 (diff | hist) . . (+73) . . RNA-seq Pipeline for Known Transcripts (added seqanswers link)
- 13:44, 3 November 2011 (diff | hist) . . (+323) . . Glucose Uptake Assay (added templates)
- 15:16, 2 November 2011 (diff | hist) . . (+415) . . N Talk:Dexamethasone Treatment of Mice (added note about protocol source) (current)
- 15:15, 2 November 2011 (diff | hist) . . (+1,052) . . N Dexamethasone Treatment of Mice (wrote initial page)
- 13:31, 21 October 2011 (diff | hist) . . (-4) . . m RNA-seq Pipeline for Known Transcripts (removed linebreak)
- 13:30, 21 October 2011 (diff | hist) . . (+959) . . RNA-seq Pipeline for Known Transcripts (added instructions for barcode splitting)
- 20:22, 3 October 2011 (diff | hist) . . (+1,165) . . N Adding New Personnel to Animal Protocol (Copied over from Shannon's Instructions) (current)
- 20:13, 3 October 2011 (diff | hist) . . (+159) . . m RNA-seq Pipeline for Known Transcripts (added link to FastQC report analysis)
- 20:12, 3 October 2011 (diff | hist) . . (+244) . . RNA-seq Pipeline for Known Transcripts (added information about using FastQC)
- 16:44, 19 September 2011 (diff | hist) . . (0) . . m Glycogen Phosphorylase Assay (typo in categories) (current)
- 16:42, 19 September 2011 (diff | hist) . . (+1,454) . . N Glycogen Phosphorylase Assay (copied protocol from alan cheng)
- 14:00, 14 September 2011 (diff | hist) . . (+120) . . RNA-seq Pipeline for Known Transcripts (added categories)
- 13:37, 14 September 2011 (diff | hist) . . (+236) . . RNA-seq Pipeline for Known Transcripts (updated formatting)
- 13:15, 14 September 2011 (diff | hist) . . (+3,169) . . N RNA-seq Pipeline for Known Transcripts (Pasted in Instructions from Richard McEachin)
- 18:39, 29 August 2011 (diff | hist) . . (+19) . . m Designing siRNA for Knockdown (updated quote number)
- 13:22, 29 August 2011 (diff | hist) . . (+13) . . m Fugene Transfection of 293T/COS Cells (added catalog number) (current)
- 13:21, 29 August 2011 (diff | hist) . . (+507) . . Fugene Transfection of 293T/COS Cells (updated protocol)
- 15:17, 24 August 2011 (diff | hist) . . (-153) . . Immunofluoresence (updated protocol)
- 14:28, 11 August 2011 (diff | hist) . . (+339) . . N Preparation of Tail Samples (for Genotyping) (Created page with 'PBND Solution: Tail Lysis Buffer -50mM KCl -10mM Tris HCl (pH~8.3) -0.1 mg/mL MgCl2, 6H20 -0.1 mg/mL Gelatin -0.45% (NP-40) Jgepal -0.45% Tween 20 Protocol 1.) Combine 200uL o...')
- 18:45, 10 August 2011 (diff | hist) . . (+14) . . Acetate Incorporation into Lipid (rewrote procedure) (current)
- 20:43, 2 August 2011 (diff | hist) . . (+2,524) . . N Antisense Oligo Knockdown in Mice (wrote initial page) (current)
- 15:03, 24 June 2011 (diff | hist) . . (+7) . . Glycogen Determination from Tissues
- 15:02, 24 June 2011 (diff | hist) . . (+379) . . Glycogen Determination from Tissues (updated details about assay)
- 18:02, 23 June 2011 (diff | hist) . . (+28) . . Rapamycin Injections (added dosage volume)
- 20:45, 18 May 2011 (diff | hist) . . (+1,019) . . N Measurement of Glycolysis and Respiration Using Seahorse (wrote initial page) (current)
- 15:00, 13 May 2011 (diff | hist) . . (+220) . . Insulin Tolerance Test (updated with new starvation and added additional information)
- 15:31, 20 April 2011 (diff | hist) . . (+846) . . N Analysing Sequencing Results with SeqMan (wrote initial page) (current)
- 14:40, 14 April 2011 (diff | hist) . . (+1) . . Surface Plasmon Resonance - Protein Lipid Interactions (updated protocol to make 300 uL of lipid to make extruding easier) (current)
- 00:42, 29 March 2011 (diff | hist) . . (+107) . . Real Time PCR From Cell Culture (added reference for calculating amounts from Ct) (current)
- 16:53, 16 March 2011 (diff | hist) . . (+318) . . Colocalization (updated protocol) (current)
- 19:55, 9 March 2011 (diff | hist) . . (+720) . . N Lipid Transfection (Created page with '==Materials== * Shuttle Kit from Echelon [http://www.echelon-inc.com/commerce/ccc1021-shuttle-pip-kits.htm] * I used C8 lipids and dissolved to 1 mM in PBS and frozen in small al...') (current)
- 21:33, 1 March 2011 (diff | hist) . . (-2) . . Acetate Incorporation into Lipid (increased from 0.1 to 10 uCi per mL.)
- 19:47, 9 February 2011 (diff | hist) . . (+177) . . Affinity Purification of Proteins - FPLC (reworded some bits of the protocol) (current)
- 19:04, 2 February 2011 (diff | hist) . . (+31) . . m Immunofluoresence (added categories)
- 19:03, 2 February 2011 (diff | hist) . . (+57) . . N Category:Yeast (Created page with 'Protocols using yeast, typically cell biology experiments') (current)
- 20:55, 31 January 2011 (diff | hist) . . (+73) . . Protein-Lipid Overlay Assay (added categories) (current)
- 20:54, 31 January 2011 (diff | hist) . . (+1,147) . . N Protein-Lipid Overlay Assay (copied protocol)
- 19:46, 12 January 2011 (diff | hist) . . (+94) . . m Glucose Uptake Assay (added categories)
- 19:47, 13 December 2010 (diff | hist) . . (0) . . Preparing an Adenoviral shRNA Clone (→Choosing a shRNA Sequence: updated XbaI compatible overhang) (current)
- 21:49, 10 December 2010 (diff | hist) . . (+33) . . Real Time PCR From Cell Culture (altered primer concentration and volumes for 384 well plate)
- 20:23, 10 December 2010 (diff | hist) . . (+82) . . m Glucose Tolerance Test (added categories)
- 17:15, 6 December 2010 (diff | hist) . . (+53) . . Real Time PCR From Cell Culture (added link to preparation of rna from tissue)
- 15:07, 6 December 2010 (diff | hist) . . (-70) . . m Genotyping Program
- 15:06, 6 December 2010 (diff | hist) . . (+497) . . N Genotyping Program (Created page with '{| border="1" !| Allele || Program|| Fragment Size(s) || Notes |- |TSC-floxed || Inoki PCR || || Two bands if heterozygote |- |Cre || Inoki PCR || || Only present if Transgene...')
- 14:50, 6 December 2010 (diff | hist) . . (+165) . . GoTaq PCR Genotyping (updated protocol and added categories) (current)
- 22:00, 2 December 2010 (diff | hist) . . (+2,189) . . N Purification of MBP Fusion Proteins (wrote initial page) (current)
- 16:17, 22 November 2010 (diff | hist) . . (+941) . . N Designing siRNA for Knockdown (wrote initial page)
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