50X TAE Buffer

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Reagents

  • 900mL ddH2O
  • 242g Tris Base
  • 57.1 mL Glacial Acetic Acid
  • 18.6g EDTA

Add Tris Base, Glacial Acetic Acid, and EDTA to 900mL water in 1L Erlenmeyer Flask and mix well.