Set up qPCR Run on Roche LightCycler
From Bridges Lab Protocols
Run Protocol
- Open LightCycler software.
- Click new experiment.
- Choose SYBR Green option from drop down and make sure that the correct block is in place (it will say 96 or 384).
- Click apply template.
- Go to templates>run templates> bridges SYBR Green 384.
- Load plate and click 'start run'.
- This protocol is set to do the following:
- Activate- 1 cycle @95 degrees for 10s.
- Amplify- 45 cycles @95, 60 and 73 degrees for 15s, 15s and 10s, respectively.
- Melt- 1 cycle @95, 40, 65, 95 and 40 degrees for 1m, 1m, 1s, continuous and 10s, respectively.
