Set up qPCR Run on Thermo Cloud and QuantStudio
From Bridges Lab Protocols
Revision as of 19:35, 29 January 2017 by Davebridges (Talk | contribs) (Wrote information for uploading plate to thermo cloud.)
Set Up Plate Layout
- Copy a plate layout file (example here) and save it as a CSV file.
- For each well you are using you need to enter the following:
- Sample Name
- Target Name
- Task (Usually UNKNOWN)
- Reporter (SYBR)
- Quencher (NFQ-MGB)
- Leave the rest of the plate blank
- For each well you are using you need to enter the following:
- Log into Thermo Cloud (https://apps.thermofisher.com/apps/dashboard using the login information for your lab (the Bridges Lab account data is located on the side of the machine.)
- Under My Apps on the right hand side click on Design and Analysis Application (qPCR).
- Click on New under Create new experiment.
- Leave the file name as the default (Date/ID)
- Change Block type to 384-Well Block.
- Change Chemistry to SYBR Green Reagents.
- Click Next or on the Method tab and change volume to 10 uL (or leave at 20 uL if you are using a total volume of 20uL/well)
- Click Next or on the Plate tab. Click on Actions then Import plate setup and select your plate layout file. Check the visual representation of your plate layout and make sure it is correct.
- Click Next or on the Run tab. The software will save your plate layout and upload it to thermo cloud.
