FDB Muscle Dissection from Mice
From Bridges Lab Protocols
This protocol is modified from section 3.1.2 of Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells and the first part of the following video with accompanying protocol DNA Electroporation, Isolation and Imaging of Myofibers | Protocol.
Materials
- Tweezers
- Standard operating scissors
- Surgical scissors
- Forceps
- Dissection stage with 7 pins
- Anesthetic
Flexor Digitorum Brevis Muscle Extraction
- Sacrifice the animal with anesthetic gas inhalation followed by cervical dislocation to ensure death.
