50X TAE Buffer
From Bridges Lab Protocols
Revision as of 14:27, 2 August 2017 by Davebridges (Talk | contribs) (→Reagents: changed category tag)
Reagents
- 900mL ddH2O
- 242g Tris Base
- 57.1 mL Glacial Acetic Acid
- 18.6g EDTA
1. Add Tris Base, Glacial Acetic Acid, and EDTA to 500mL water in 1L Erlenmeyer Flask and mix well by swirling the flask. 2. Add remaining 400mL water and mix on stir plate (or my swirling).
