Phosphate Buffered Saline

From Bridges Lab Protocols
Revision as of 21:31, 25 January 2018 by Davebrid (talk | contribs) (Created page with "==Recipe== * Start with 800 ml of distilled water: * Add 8 g of NaCl. * Add 0.2 g of KCl. * Add 1.44 g of Na<sub>2</sub>HPO<sub>4</sub>. * Add 0.24 g of KH<sub>2</sub>PO<sub>4...")
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Recipe

  • Start with 800 ml of distilled water:
  • Add 8 g of NaCl.
  • Add 0.2 g of KCl.
  • Add 1.44 g of Na2HPO4.
  • Add 0.24 g of KH2PO4.
  • Adjust the pH to 7.4 with HCl.
  • Add distilled water to a total volume of 1 liter.
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