Phosphate Buffered Saline
From Bridges Lab Protocols
Revision as of 21:55, 25 January 2018 by Davebridges (Talk | contribs)
Recipe
- Start with 400ml of distilled water:
- Add 4 g of NaCl.
- Add 0.72 g of KCl.
- Add 0.72 g of Na2HPO4.
- Add 0.12 g of KH2PO4.
- Adjust the pH to 7.4 with HCl.
- Add distilled water to a total volume of 500 mL.