Cre PCR

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PCR Program

  • Cre (F) GCATT ACCGG GCAAC GAGTG ATGAG
  • Cre (R) GAGTG AACGA ACCTG GTCGA AATCA GTGCG
  • 95C 1min
  • 35 Cycles of:
    • 95C 10s
    • 55C 30s
    • 72C 45s
  • 72C 5min
  • 4C forever
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