Deparaffinizing Histology Slides

From Bridges Lab Protocols
Revision as of 21:52, 4 June 2020 by Reddj (Talk | contribs) (added SOP requirements)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

SOP

Materials

  1. Xylene
  2. 100% Ethanol
  3. 95% Ethanol

Method

  • Use slide rack/holder
  1. Xylene: 2 x 3 minutes
  2. Xylene 1:1 with 100% Ethanol: 3 minutes
  3. 100% Ethanol: 2 x 3 minutes
  4. 95% Ethanol: 3 minutes
  5. 70% Ethanol: 3 minutes
  6. 50% Ethanol: 3 minutes
  7. Running cold tap water to rinse

Keep the slides in the tap water until ready to perform staining protocol.

https://www.abcam.com/ps/pdf/protocols/ihc_p.pdf

Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php?title=Deparaffinizing_Histology_Slides&oldid=1586"