Genotyping Program

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Allele Program Fragment Size(s) Notes
TSC-floxed Cre PCR Two bands if heterozygote
Cre Cre PCR Only present if Transgene Positive (cannot detect homozygosity at transgene)
Vac14 Cre PCR Two bands if heterozygote
TC10b TC10b KO - 500bp, WT-1kb Two bands if heterozygote
ob/ob Genotyping ob/ob KO - 123bp, WT-104kb, control 191 bp Two reactions, see ob/ob Genotyping
MTM1 MTM1 Mutant-575, WT-714 Separate Reactions for WT and KO
CAP CAP Two bands if heterozygote, upper band for KO
Vinexin Vinexin
[[nr3c1 Genotyping|