4EBP1/2 Genotyping

PRIMERS

1. '4EBP1

• 4EBP1- Fwd: GAT GGA GTG TCG GAA CTC ACC (1X)
• 4EBP1- Rev: GAC CTG GAC AGG ACT CAC CGC (2X)
• 4EBP1/2- Neo Fwd: GCA TCG AGC GAG CAC GTA CTC (1X)

1. 4EBP2

• 4EBP2-Fwd: GGT GGG ACT GTC GGT CTT CTG (1X)
• 4EBP2-Rev: CAG CAC CTG GTC ATA GCC GTG (2X)
• 4EBP1/2- Neo Fwd: GCA TCG AGC GAG CAC GTA CTC (1X)

Make primers at 1uM (Fwd1 + Rev1, Neo Fwd1/2 + Rev1, Fwd2 + Rev2, and Neo Fwd1/2 + Rev2). PCR 4EBP1 and 4EBP2 primers separately.