PCR Analysis of Tail DNA

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see Genotyping Details for strain specific details

Materials

Protocol

Use the following Volumes per Reaction:

Buffer: 4 uL of 5X Go-Taq buffer ("Molecular Biology Stuff" box in freezer)

Forward Primer: .4ul

Reverse Primer: .4ul

dNTPs: .4uL of 2 mM ("Molecular Biology Stuff" box in freezer)

Sterile water: 13.6 uL

Polymerase Go-Taq: 1 uL (6th floor in Genotype Yellow Box in freezer)

Template: 1 uL


see Preparing an Agarose Gel for details on preparing a DNA gel

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