Buffer/RIPA

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RIPA Buffer (for 10mL lysis buffer)

Final Concentration per 10 mL Stock Location
Tris pH7.4 50mM 500uL 1M
Na Deoxycholate 0.25% 250uL 10%
NP-40 1% 1mL 10%
NaCl 150mM 375uL 4M
EDTA 1mM 20uL 0.5M
NaVO3 1mM 100uL 1M Biochemical Stocks (-20)
NaF 5mM 100uL 500mM
NaPPi 25 mM 1 mL 250mM
Protease Inhibitors 1 mini tablet 4C
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