Bradford Assay

Materials

• BioRad Protein Assay Dye Reagent Concentrate cat#500-0006
• Disposable Plastic Cuvette

Protocol

1. Dilute reagent 5X in water, stable for 2-3 weeks
2. Pipet 1 mL into disposable plastic cuvette
3. Add 1-10 uL of protein sample, cover with parafilm and mix
4. Let sit 5-10 min to react
5. Set spectrophotometer as follows:
   1. Go to protein assay then Bradford assay
   2. Set formula, then select more
   3. Set b=0.045 (or determine slope)
   4. Set dilution to be 1/vol (ie 0.1 for 10 uL)
   5. Blank then measure samples, absorbance must be less than 0.9
   6. Print (hit Recall, then enter, then print) and attach to experiment

Reference

• Wikipedia: Bradford Protein Assay
• PMID 942051