Rab5 FRET Assay

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Materials

mCitrine-Rab5BD (from EEA1/NT)
mCFP-Rab5A
Acid etched Coverslips (see Acid Etching Coverslips)
Cells of interest

Protocol

Plate out ~150 000 cells per well in a 6 well dish with an acid etched coverslip
The next morning transfect cells with Fugene, Lipofectamine or other protocol
That evening replace with fresh media (and serum starve if doing insulin/growth factor stimulation

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