Analysing Sequencing Results with SeqMan

1. Open Seqman (under DNAStar Lasergene)
2. Download all sequence files from FTP site to a working folder (ftp://seqcore.brcf.med.umich.edu/)
3. In SeqMan go to Sequence -> Add...
4. In your working folder select AB1 files and then Add then Done
5. If adding a reference sequence click Add Sequence... button then enter the nucleotide accession number and save this to your working folder.
6. Click Assemble button on the new window
7. A contig should be assembled on the window to the right. Double click on this.
8. Scan through the assembled contig to check for errors. Ignore errors only at ends of one sequencing run.
9. To see the chromatogram click on the grey triangle at the left of the sequence
10. Save the assembly as a SeqMan file.