Genotyping Program
From Bridges Lab Protocols
Allele | Program | Fragment Size(s) | Notes |
---|---|---|---|
TSC-floxed | Inoki PCR | Two bands if heterozygote | |
Cre | Inoki PCR | Only present if Transgene Positive (cannot detect homozygosity at transgene) | |
Vac14 | Inoki PCR | Two bands if heterozygote | |
TC10b | TC10b | KO - 500bp, WT-1kb | Two bands if heterozygote |
CAP | CAP | Two bands if heterozygote |