MTM1 Genotyping

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See http://jaxmice.jax.org/protocolsdb/f?p=116:2:3785778730065538::NO:2:P2_MASTER_PROTOCOL_ID,P2_JRS_CODE:8261,018153

Primers

  • MTM1 Fwd: GGC CCC ATT AAG GGA AGA GT
  • MTM1 WT Rev: GAT CTT CTG GGA AAG GCA CA
  • MTM1 Mut Rev: GCC AGA GGC CAC TTG TGT AG

PCR Program

  • 94 for 2 min
  • 10 cycles of:
    • 94 for 20sec
    • 65 for 15sec with -0.5 C per cycle
    • 68 for 10sec
  • 28 cycles of:
    • 94 for 15sec
    • 60 for 15sec
    • 72 for 10sec
  • 72 for 1 min
  • 4 hold

Expected Results

File:MTM1 Genotyping Result.jpg

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