TBST

From Bridges Lab Protocols

Revision as of 14:52, 12 April 2013 by Davebridges (Talk | contribs) (copied recipe from saltiel lab buffers)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Jump to: navigation, search

10X TBS Buffer

To make 1X TBS, dilue 10X with water, and add 10 mL/10L Tween 20 (Sigma P7949-500ml)

Component	Weight per 10 L	Catalog Number
Tris Base	606 g	Sigma T6066-5KG
NaCl	877 g	Sigma S7653-5KG
HCl	360 mL	Sigma 258148-2.5L

Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php?title=TBST&oldid=748"