TC10b Genotyping

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Primers

  • TC10b - PDFor1: GGAGTCTATGGACATGAGAG
  • TC10b - PD4-43: GATCATGACTAGCATGGAGTAG
  • TC10b - PDHomoFB: GTCTATGGACATGAGAGTTTC
  • TC10b - NEO3A: GCAGCGCATCGCCTTCTATC

Genotyping Program

  • 94 2min
  • 50 cycles of:
    • 94 30s
    • 56 30s
    • 72 4min
  • 72 7min
  • 4 forever

Results

WT allele ~ 1kb, KO allele ~ 500bp HET should get two bands

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