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Designing and Generating CRISPR-Cas Mutants
From Bridges Lab Protocols
Revision as of 16:19, 1 March 2014 by
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(added notes about diluting insert)
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Do I need to dilute the insert 250X
Tried using undiluted insert and 250X diluted insert. Got zero colonies for undiluted inserts and 26 colonies for each of the diluted inserts. (
Davebridges
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