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Materials
- 0.9 % Saline solution
- 10% formalin solution
- Avertin
- Isofluorane
- 30 ½ gauge needle
- 1 mL syringe
- Peristaltic pump
- Collecting dish
- Surgical stage/platform
- Make sure this is level with the edge of the collecting dish to allow for the need to rest during the procedure
Surgical tools:
- Dissecting scissors
- Forceps
- Clamp scissors
- Standard scissors
Avertin Preparation
- Taken from: http://web.jhu.edu/animalcare/rdf/avertin.html
- Stock Solution (1.6 g/ml)
- Mix 25 g avertin and 15.5 ml tert-amyl alcohol at room temperture for ~12 hours in a dark bottle.
- Can store stock solutions at room temperature for one year.
- Working solution (20 mg/ml)
- Mix 0.5 ml avertin stock solution and 39.5 ml 0.9% saline in dark/foil covered container.
- Filter solution through 0.2 micron filter into a dark/foiled covered container.
- Store solution at 4 degree C.
- Replace working solution each month.
- Notes:
- Avertin is light sensitive.
- Degredation products are lethal.
- Always store at 4 degree C
- Do NOT use a solution that is yellow or contains a precipitate.
- Avertin is lipid soluble and may require a larger dose.