Rapamycin Injections

SOP

• Safety and Animal Training
• SOP - Needle Capping
• SOP - Rapamycin

Materials

• Tween 80 - Sigma P1754
• PEG 400 Liquid - Sigma P3265

Preparation

• Weigh out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip. This is 5.2% PEG/Tween
• Dissolve rapamycin at 20 mg/mL (0.0219 M) in ethanol (prepare as a 1 mL aliquot and store at -20 C).
• Add 5 uL of 20 mg/mL RAPA in 1 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (109 uM; use this for 1 week).
• IP inject 10 uL/g body weight (1 mg/kg)

Alternate Rapamycin Injection Regimen

• Phenotypes seem to be different with rapamycin as treated by Lamming et al.
• This protocol is from Lamming et al http://dx.doi.org/10.1126/science.1215135

rapamycin treatment was performed by injecting 8 to 10 week old mice intraperitoneally once daily with either 1 mg/kg rapamycin suspended in 0.9% NaCl and 2% ethanol at a concentration of 0.5 mg/mL (547 μM), or vehicle only for 14-28 days.