Difference between revisions of "Affinity Purificatoin of Antibodies (Coupling to Activated CH Sepharose 4B)"

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(Created page with ''''Materials''' #0.1M NaBicarbonate (0.8401g in 100mL ddH<sub>2</sub>) and 0.5MNaCl (2.922g in 100mL ddH<sub>2</sub>)pH 8.0.')
 
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'''Materials'''
 
'''Materials'''
#0.1M NaBicarbonate (0.8401g in 100mL ddH<sub>2</sub>) and 0.5MNaCl (2.922g in 100mL ddH<sub>2</sub>)pH 8.0.
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#0.1M NaBicarbonate (0.8401g in 100mL ddH<sub>2</sub>O) and 0.5MNaCl (2.922g in 100mL ddH<sub>2</sub>O)at pH 8.0.
 +
#50mM Sodium Acetate (0.41g in 100mL ddH<sub>2</sub>O)at pH 4.0
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#50mM Tris (0.61g in 100mL ddH<sub>2</sub>O) and .5M NaCl (2.922g in 100mL ddH<sub>2</sub>O) at pH 8.0
 +
#0.4M glycine (3.004g in 100mL ddH<sub>2</sub>O)at pH 1.8.
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'''Procedure'''
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Protein must be extensively dialysed in PPBS to remove any amine group from the sample.
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#

Revision as of 13:17, 20 May 2009

Materials

  1. 0.1M NaBicarbonate (0.8401g in 100mL ddH2O) and 0.5MNaCl (2.922g in 100mL ddH2O)at pH 8.0.
  2. 50mM Sodium Acetate (0.41g in 100mL ddH2O)at pH 4.0
  3. 50mM Tris (0.61g in 100mL ddH2O) and .5M NaCl (2.922g in 100mL ddH2O) at pH 8.0
  4. 0.4M glycine (3.004g in 100mL ddH2O)at pH 1.8.

Procedure Protein must be extensively dialysed in PPBS to remove any amine group from the sample.