TORC1 Kinase Buffer
From Bridges Lab Protocols
mTORC1 Kinase Buffer (for 10mL lysis buffer)
| Final Concentration | per 10 mL | Stock | Location | |
|---|---|---|---|---|
| HEPES, pH 7.4 | 25 mM | 250 uL | 1M | 4C |
| KCl | 50 mM | 500 uL | 1M | Shelf |
| MgCl2 | 10 mM | 100 uL | 1M | Shelf |
| ATP | 250 uM | 25 uL | 100mM | -20 |
see PMID 19200882
