Amitryptyline Treatment of Mice
From Bridges Lab Protocols
We use Amitiptyline from Sigma (A8404).
For drinking water administration ~10 mg/kg/day
We have approval for 1-100 mg/kg/day. Still need to pilot these effects
- Makes 1L of drinking water
- dissolve 0.060g in 1L water
Calculation
- 10 mg drug/kg/day because mice drink ~5mL /day so want 2 mg drug/kg/mL
- average mouse weighs 0.03kg so want 0.06 mg drug/mL or 60 mg/L
- This assumes that the mice are about 30g and they drink 5mL/day. The volume drank per cage should be monitored.
