High Salt Immune Complex Wash Buffer
From Bridges Lab Protocols
| Chemical | Final Concentration | Per 15mL | Stock | Location |
|---|---|---|---|---|
| SDS | 0.1% | 150uL | 10% | Solutions Shelf |
| Triton X-100 | 1% | 1.5mL | 10% | Solutions Shelf (borrowed from Seo Lab to make stock) |
| EDTA | 2mM | 60uL | 500mM | Solutions Shelf |
| Tris | 20mM | 300uL | 1M | Solutions Shelf |
| NaCl | 500mM | 1.875mL | 4M | Solutions Shelf |
