Matrigel Basement Membrane Matrix
From Bridges Lab Protocols
STORAGE
Store unopened an aliquoted Matrigel at -20°C. DO NOT STORE IN FROST-FREE FREEZER. KEEP FROZEN.
RECONSTRUCTION AND USE
Color variation may occur between frozen and thawed vials. Variation does not affect product efficacy, and will disappear upon equilibration with 5% Carbon dioxide.
1. Thaw on ice in the back of a 4°C refrigerator, overnight.
2. Once thawed, swirl to ensure even distribution.
3. Keep on ice at all times-incuding while aliquoting.
4. Handle with STERILE technique. Place vial in sterile area, spray top with 70% EtOH and air dry.
5. Divide into 100 uL aliquots in 1.5mL Eppendorf tubes.
- Pre-cool pipette tips.
- Change tips whenever Matrigel is clogging the tip and/or causing the pipette to aspirate inaccurately.
