Nr3c1 Genotyping
From Bridges Lab Protocols
PRIMERS
nr3c1
- Fwd: AAACAGGGTTATGCTTGGCA
- Rev: TGCCTGCTAGGCAAATGATCT
Cre
- Fwd: GCATT ACCGG GCAAC GAGTG ATGAG
- Rev: GAGTG AACGA ACCTG GTCGA AATCA GTGCG
Make nr3c1 primer dilution at 1uM (10uL each primer + 980uL water) and Cre at .4uM. Primers in Genotyping Box are marked with a star.
PCR PROTOCOL
- 94 °C 2 min
- 10 cycles of:
- 94 °C 20 s
- 65 °C 15 s
- 68 °C 10 s
- 28 cycles of:
- 94 °C 15 s
- 60 °C 15 s
- 72 °C 10 s
- 72 °C 2 min
