User contributions
This user is currently blocked. The latest block log entry is provided below for reference:
- 14:13, 1 April 2014 (diff | hist) . . (+491) . . N BDNF PCR (Created page with "'''BDNF Primers''' ---- * oIMR0133 ---- ATG AAA GAA GTA AAC GTC CAC (Common) * oIMR0113 ---- CCA GCA GAA AGA GTA GAG GAG (WT Reverse) * oIMR01694 --- GGG AAC TTC CTG ACT AG...") (current)
- 14:03, 1 April 2014 (diff | hist) . . (+13) . . Main Page (Added BNDF)
- 15:03, 19 February 2014 (diff | hist) . . (-1) . . Rapamycin Injections
- 15:09, 1 November 2013 (diff | hist) . . (+990) . . N Paraffin Embedding of Tissue Samples (Created page with " == Protocol == #After harvesting tissue from animal, place into pathology cassette and fix in 4% formaldehyde, 4% paraformaldehyde or 4% formalin O/N #After fixation, begin d...")
- 14:50, 1 November 2013 (diff | hist) . . (+42) . . Main Page (→Tissue Preparation)
- 13:56, 13 September 2013 (diff | hist) . . (0) . . Fly Food Protocol (→Cooking Insturctions) (current)
- 17:37, 3 September 2013 (diff | hist) . . (+7) . . Fly Food Protocol (→Cooking Insturctions)
- 17:36, 3 September 2013 (diff | hist) . . (+2,037) . . N Fly Food Protocol (Created page with " == Quantities == '''Summer''' #900mL - Corn Syrup #202.4g - Yeast #117.2g - Soy Flour #856g - Corn Meal #67.6g - Agar #15L - Water #65mL - Propionic Acid...")
- 16:29, 3 September 2013 (diff | hist) . . (+23) . . Main Page (→Fly Stocks)
- 15:23, 7 August 2013 (diff | hist) . . (+17) . . RIPA Buffer (→RIPA Buffer (for 10mL lysis buffer))
- 15:22, 7 August 2013 (diff | hist) . . (+32) . . RIPA Buffer (→RIPA Buffer (for 10mL lysis buffer))
- 15:21, 7 August 2013 (diff | hist) . . (0) . . RIPA Buffer (→RIPA Buffer (for 10mL lysis buffer))
- 16:09, 6 August 2013 (diff | hist) . . (+1) . . Maintenance of 18C Fly Stocks (→=Maintenance) (current)
- 16:09, 6 August 2013 (diff | hist) . . (+397) . . N Maintenance of 18C Fly Stocks (Created page with "==Location== Drosophila stock vials can be found in the 18C cooler of the Reiter Laboratory ==Maintenance= *Drosophila stocks are to be flipped every 3 weeks *When flipping t...")
- 16:04, 6 August 2013 (diff | hist) . . (0) . . Main Page (→Fly Stocks)
- 16:02, 6 August 2013 (diff | hist) . . (+360) . . N Maintanence of 18C Fly Stocks (Created page with "==Location== Drosophila stocks can be found in the 18C cooler of the Reiter Lab ==Maintenance== *Drosophila stocks are to be "flipped" every 3 weeks *Always flip the oldest o...") (current)
- 15:55, 6 August 2013 (diff | hist) . . (+51) . . Main Page (→Mouse/Fly Protocols)
- 15:54, 6 August 2013 (diff | hist) . . (+4) . . Main Page (→Mouse Protocols)
- 14:29, 1 August 2013 (diff | hist) . . (+102) . . Triglyceride Assay from Drosophila (German Method) (→Protocol) (current)
- 21:26, 31 July 2013 (diff | hist) . . (+1,305) . . Triglyceride Assay from Cells and Tissues (→Protocol)
- 21:12, 31 July 2013 (diff | hist) . . (+412) . . N Triglyceride Assay from Drosophila (German Method) (Created page with "==Protocol== #Use 5 female or 8 Male flies for Assay #Homogenize flies (3 min @ 40Hz) in 1mL of .05% Tween (from Tween-20 stock) #Heat samples in 70C waterbath for 5 minutes #...")
- 21:11, 31 July 2013 (diff | hist) . . (-1,406) . . Triglyceride Assay from Cells and Tissues (→Protocol: Creation of Protocol)
- 21:05, 31 July 2013 (diff | hist) . . (+56) . . m Main Page (→Protein Purification)
- 21:27, 26 July 2013 (diff | hist) . . (+13) . . m Preparation of Protein Lysates from Mouse Tissues (→Protocol)
- 21:23, 26 July 2013 (diff | hist) . . (+266) . . m Bradford Assay (→Protocol: Added Protein Lysate)
- 13:55, 26 July 2013 (diff | hist) . . (+39) . . PCR Analysis of Tail DNA (→Protocol)
- 15:00, 25 July 2013 (diff | hist) . . (+247) . . m PCR Analysis of Tail DNA (→Protocol: Master Mix)
- 19:39, 11 July 2013 (diff | hist) . . (+133) . . m Western Blotting (→Protocol: LiCor Addition)
- 19:34, 11 July 2013 (diff | hist) . . (+2) . . m Transfer Buffer (→10X Transfer Buffer: Changed from 10L total to 4L total) (current)
- 19:33, 11 July 2013 (diff | hist) . . (-6) . . m Western Blotting (→Protocol: Changed wash times)
- 17:10, 28 June 2013 (diff | hist) . . (0) . . m Triglyceride Assay from Cells and Tissues (→Protocol: Volume of butanol mixture)
- 17:18, 26 June 2013 (diff | hist) . . (+31) . . m Preparation of Tail Samples (for Genotyping) (→Protocol: Proteinase K)
- 15:29, 19 June 2013 (diff | hist) . . (-55) . . m Triglyceride Assay from Cells and Tissues (Deletion of unnecessary information (1/10 Glycerol Dilution))
- 15:24, 19 June 2013 (diff | hist) . . (-466) . . Triglyceride Assay from Cells and Tissues (updated volumes and lysis procedure)
