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• PCR Amplification of DNA
  1 KB (162 words) - 16:57, 8 June 2020
• PCR Analysis of Tail DNA
  # Tail digest DNA *[[PCR Amplification of DNA]]
  773 bytes (111 words) - 17:00, 8 June 2020
• DNA Preparation from Tail Clip
  1 KB (161 words) - 17:58, 5 June 2009
• Cesium Chloride Preparation of DNA
  #Resuspend DNA in 6 mL of distilled water. Add 10g of CsCl and dissolve.
  1 KB (167 words) - 22:03, 17 January 2012
• PCR Amplification of DNA (KOD Polymerase)
  1 KB (179 words) - 21:24, 3 November 2009
• Rapid Isolation of Genomic DNA from Yeast
  [[Category:DNA]] ===Yeast DNA Prep Buffer===
  1 KB (197 words) - 16:32, 21 February 2012
• Verifying Kan Deletion Mutants from Genomic DNA
  [[Category:DNA]] ...Yeast. See [[Rapid Isolation of Genomic DNA from Yeast]]. Use 1µl of the DNA in your PCR reaction.
  1 KB (248 words) - 16:39, 21 February 2012
• Sending Samples for DNA Sequencing
  # Select '''Enter Individual DNA Sequencing Requests''' * 1 uL of template (unless the plasmid DNA concentration is >500 ng/uL in which case dilute it to 50-300 ng/uL
  646 bytes (108 words) - 18:34, 5 March 2014
• Extraction of DNA from TRIZOL preparations
  This protocol is for extracting DNA from TRIZOL preparations after the RNA-containing upper phase has been comp ...sion or use the shaker. '''NEVER''' vortex samples, as it will destroy the DNA.
  3 KB (417 words) - 17:42, 25 September 2015
• Surveyor Assay for Genomic DNA Mutations
  ===DNA Extraction=== ** 1 uL of the Extracted DNA from the previous step.
  2 KB (262 words) - 20:02, 13 July 2015
• Spill Response Procedures for Infectious agents and Recombinant DNA
  6 KB (867 words) - 19:50, 13 September 2019

Page text matches

• Main Page
  *[[PCR Amplification of DNA]] *[[Cesium Chloride Preparation of DNA]]
  4 KB (484 words) - 12:10, 15 August 2016
• Transformation of Bacteria
  *DNA of interest (1 uL for plasmid, 5 uL for cloning/mutagenesis) #Add DNA to cells and mix by tapping
  598 bytes (104 words) - 15:44, 12 February 2014
• Restriction Enzyme Based Cloning
  #Add 1 uL T4 DNA Ligase (Invitrogen). ##Add DNA and mix gently
  1 KB (227 words) - 14:40, 7 May 2009
• PCR Analysis of Tail DNA
  # Tail digest DNA *[[PCR Amplification of DNA]]
  773 bytes (111 words) - 17:00, 8 June 2020
• Electroporation of 3T3-L1 Adipocytes
  *DNA: CsCl Purified or siRNA (resuspended to 0.1 nmol/uL with 200 uL water) #500 uL of cells is mixed with 50-200 ug of DNA (or 5 uL siRNA) in a 0.4 cm cuvette. These volumes are for half of a final
  1 KB (236 words) - 18:45, 17 February 2010
• Check Clones for Correct Mutation
  #Paste dna sequence in top box
  427 bytes (73 words) - 13:24, 27 May 2009
• Cesium Chloride Preparation of DNA
  #Resuspend DNA in 6 mL of distilled water. Add 10g of CsCl and dissolve.
  1 KB (167 words) - 22:03, 17 January 2012
• Fugene Transfection of 293T/COS Cells
  * DNA - typically transfect 50-1000 ng DNA per well ##Per ug of DNA need 3 uL Fugene.
  1 KB (198 words) - 13:22, 29 August 2011
• Lipofectamine Mediated Knockdown
  *OptiMEM(in uL, equal for DNA and Lipofectamine) = Lipofectamine(in uL) * 50 ##Combine the two volumes of OptiMEM/DNA and OptiMEM/Lipofectamine (should be 200uL/well).
  1 KB (148 words) - 16:39, 18 December 2009
• Designing and Preparing dsRNA
  *Load 1 and 3 uL of diluted RNA, plus 4 or 8 uL of 1 kB Plus DNA Ladder
  2 KB (290 words) - 14:31, 1 October 2009
• Yeast Transformation
  *Herring testes DNA (Clontech Cat# 17401336) #Boil hering tested DNA for 5 min at 95C then cool on ice.
  2 KB (292 words) - 17:14, 22 December 2009
• Lipofectamine Plasmid Transfection
  *Calculate DNA to transfect *Lipofectamine(in uL) = DNA(in ug) * 2.5
  1 KB (155 words) - 21:00, 11 July 2012
• Purification of GST-HA-S6K
  *GST-HA-S6K1 plasmid. Prepare by [[Cesium Chloride Preparation of DNA]]. Need 500 ug per 10 plates of cells #Combine 500 ug DNA with 10 mL OptiMEM, and separately 700 uL Lipofectamine 2000 with 10 mL Opt
  1 KB (185 words) - 19:59, 20 September 2012
• Yeast Transfection (Small Scale)
  *Herring testes carrier DNA. Boil 20min then place on ice before use #Place Herring DNA on heating block at 95C for 20min then immediately on ice before use.
  2 KB (274 words) - 15:02, 14 December 2009
• Preparing an Adenoviral shRNA Clone
  Order primers through IDT-DNA
  2 KB (353 words) - 19:47, 13 December 2010
• Generating and Amplifying Adenoviral Constructs
  ===Linearization and Purification of Shuttle DNA=== #Digest 0.2-0.5 ug plasmid DNA in 100 uL volume with 30-100U of PmeI overnight at 37C. If desired check a
  6 KB (872 words) - 20:56, 11 July 2012
• Genotyping Program
  [[ Category:DNA]]
  2 KB (297 words) - 14:32, 23 July 2021
• Preparation of Tail Samples (for Genotyping)
  [[Category: DNA]]
  1 KB (184 words) - 15:20, 21 May 2018
• Rapid Isolation of Genomic DNA from Yeast
  [[Category:DNA]] ===Yeast DNA Prep Buffer===
  1 KB (197 words) - 16:32, 21 February 2012
• Verifying Kan Deletion Mutants from Genomic DNA
  [[Category:DNA]] ...Yeast. See [[Rapid Isolation of Genomic DNA from Yeast]]. Use 1µl of the DNA in your PCR reaction.
  1 KB (248 words) - 16:39, 21 February 2012

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