YPD Media and Agar

Materials

• YPD Medium (Clontech 630409)
• Agar (if making plates)
• Adenine Hemisulfate if making YPDA. Dissolve to 0.2% and autoclave.

Preparation

• Weigh out 5g/100 mL of media
• If making plates, add 2g/100mL of bacto-agar
• Stir to dissolve and check that pH is at 5.8
• Autoclave for 15 min at 121C using the liquid cycle
• If making YPDA plates let cool to ~55C add 1.5 mL/100mL adenine
• If making rapamycin plates let cool to ~55C add desired amount of rapamycin
• Pour plates, let dry and store in the cold room