View source for Matrigel Basement Membrane Matrix

==STORAGE==

Store unopened an aliquoted Matrigel at -20°C.  DO NOT STORE IN FROST-FREE FREEZER.  KEEP FROZEN.


==RECONSTRUCTION AND USE==

Color variation may occur between frozen and thawed vials.  Variation does not affect product efficacy, and will disappear upon equilibration with 5% Carbon dioxide.


1.  Thaw on ice in the back of a 4°C refrigerator, overnight.

2.  Once thawed, swirl to ensure even distribution.

3.  Keep on ice at all times-incuding while aliquoting.

4.  Handle with STERILE technique.  Place vial in sterile area, spray top with 70% EtOH and air dry.

5.  Divide into 100 uL aliquots in 1.5mL Eppendorf tubes.

*Pre-cool pipette tips.

*Change tips whenever Matrigel is clogging the tip and/or causing the pipette to aspirate inaccurately.