Difference between revisions of "Matrigel Basement Membrane Matrix"
From Bridges Lab Protocols
(Created page with "==STORAGE== Store unopened an aliquoted Matrigel at -20°C. DO NOT STORE IN FROST-FREE FREEZER. KEEP FROZEN. ==RECONSTRUCTION AND USE== *Color variation may occur betwee...") |
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==RECONSTRUCTION AND USE== | ==RECONSTRUCTION AND USE== | ||
| − | + | Color variation may occur between frozen and thawed vials. Variation does not affect product efficacy, and will disappear upon equilibration with 5% Carbon dioxide. | |
| − | 1. Thaw on ice in the back of a 4°C refrigerator, overnight. | + | |
| − | 2. Once thawed, swirl to ensure even distribution. | + | 1. Thaw on ice in the back of a 4°C refrigerator, overnight. |
| − | 3. Keep on ice at all times-incuding while aliquoting. | + | |
| − | 4. Handle with STERILE technique. Place vial in sterile area, spray top with 70% EtOH and air dry. | + | 2. Once thawed, swirl to ensure even distribution. |
| − | 5. | + | |
| + | 3. Keep on ice at all times-incuding while aliquoting. | ||
| + | |||
| + | 4. Handle with STERILE technique. Place vial in sterile area, spray top with 70% EtOH and air dry. | ||
| + | |||
| + | 5. Divide into 100 uL aliquots in 1.5mL Eppendorf tubes. | ||
| + | |||
| + | *Pre-cool pipette tips. | ||
| + | |||
| + | *Change tips whenever Matrigel is clogging the tip and/or causing the pipette to aspirate inaccurately. | ||
Latest revision as of 15:15, 28 April 2017
STORAGE
Store unopened an aliquoted Matrigel at -20°C. DO NOT STORE IN FROST-FREE FREEZER. KEEP FROZEN.
RECONSTRUCTION AND USE
Color variation may occur between frozen and thawed vials. Variation does not affect product efficacy, and will disappear upon equilibration with 5% Carbon dioxide.
1. Thaw on ice in the back of a 4°C refrigerator, overnight.
2. Once thawed, swirl to ensure even distribution.
3. Keep on ice at all times-incuding while aliquoting.
4. Handle with STERILE technique. Place vial in sterile area, spray top with 70% EtOH and air dry.
5. Divide into 100 uL aliquots in 1.5mL Eppendorf tubes.
- Pre-cool pipette tips.
- Change tips whenever Matrigel is clogging the tip and/or causing the pipette to aspirate inaccurately.
