Difference between revisions of "ChIP Elution Buffer"

From Bridges Lab Protocols
Jump to: navigation, search
(Created page with "10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water.")
 
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
 +
 +
{| class="wikitable"
 +
|-
 +
! Chemical !! Final Concentration !! Per 5mL !! Stock !! Location
 +
|-
 +
| SDS || 1% || 500uL || 10% || Solutions Shelf
 +
|-
 +
| NaHCO3 || 0.1M || 42g || Powder || Chemical Shelf
 +
|}
 +
 +
OR you can make this way but the stocks for this are not made up, it ends up being the same final concentrations
 
10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water.
 
10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water.

Latest revision as of 22:09, 9 January 2018

Chemical Final Concentration Per 5mL Stock Location
SDS 1% 500uL 10% Solutions Shelf
NaHCO3 0.1M 42g Powder Chemical Shelf

OR you can make this way but the stocks for this are not made up, it ends up being the same final concentrations 10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water.