User contributions
This user is currently blocked. The latest block log entry is provided below for reference:
(newest | oldest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
- 15:23, 10 May 2018 (diff | hist) . . (+48) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads) (current)
- 15:21, 10 May 2018 (diff | hist) . . (+6) . . Chromatin Immunoprecipitation (→Prior to starting this section:)
- 15:20, 10 May 2018 (diff | hist) . . (+18) . . Chromatin Immunoprecipitation (→Reverse Crosslinks of Protein/DNA Complexes to Free DNA)
- 17:34, 10 January 2018 (diff | hist) . . (+2) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 17:33, 10 January 2018 (diff | hist) . . (+122) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 22:13, 9 January 2018 (diff | hist) . . (-8) . . Chromatin Immunoprecipitation (→Prior to starting this section:)
- 22:09, 9 January 2018 (diff | hist) . . (0) . . ChIP Elution Buffer (current)
- 22:09, 9 January 2018 (diff | hist) . . (+313) . . ChIP Elution Buffer
- 21:41, 9 January 2018 (diff | hist) . . (+6) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 21:41, 9 January 2018 (diff | hist) . . (+2) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 21:32, 9 January 2018 (diff | hist) . . (+57) . . Chromatin Immunoprecipitation (→Before You Start)
- 21:29, 9 January 2018 (diff | hist) . . (+337) . . Dilution Buffer (current)
- 20:17, 9 January 2018 (diff | hist) . . (+71) . . N ChIP Elution Buffer (Created page with "10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water.")
- 20:15, 9 January 2018 (diff | hist) . . (+79) . . N Dilution Buffer (Created page with "0.01% SDS, 1.1% Triton X- 100, 1.2mM EDTA, 16.7mM Tris-HCl, pH 8.1, 167mM NaCl.")
- 20:15, 9 January 2018 (diff | hist) . . (+22) . . Chromatin Immunoprecipitation (→Buffers and Solutions Needed)
- 20:12, 9 January 2018 (diff | hist) . . (+139) . . Chromatin Immunoprecipitation (→Buffers and Solutions Needed)
- 20:11, 9 January 2018 (diff | hist) . . (-79) . . Chromatin Immunoprecipitation (→Buffers and Solutions Needed)
- 20:09, 9 January 2018 (diff | hist) . . (+196) . . N TE Buffer (Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15mL !! Stock !! Location |- | Tris|| 10mM|| 150uL || 1M || Solutions Shelf |- | EDTA || 1mM || 30uL || 500mM...") (current)
- 20:07, 9 January 2018 (diff | hist) . . (+4) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 20:05, 9 January 2018 (diff | hist) . . (+402) . . N High Salt Immune Complex Wash Buffer (Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15mL !! Stock !! Location |- | SDS || 0.1% || 150uL || 10% || Solutions Shelf |- | Triton X-100 || 1% || 1.5mL...") (current)
- 19:59, 9 January 2018 (diff | hist) . . (+4) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 19:58, 9 January 2018 (diff | hist) . . (+6) . . Low Salt Immune Complex Wash Buffer (current)
- 19:54, 9 January 2018 (diff | hist) . . (+355) . . N LiCl Immune Complex Wash Buffer (Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location |- | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf |- | NP40 || 1% || 1.5mL || 1...") (current)
- 19:54, 9 January 2018 (diff | hist) . . (+4) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 19:53, 9 January 2018 (diff | hist) . . (+4) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 19:52, 9 January 2018 (diff | hist) . . (-21) . . Low Salt Immune Complex Wash Buffer
- 19:48, 9 January 2018 (diff | hist) . . (+376) . . N Low Salt Immune Complex Wash Buffer (Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location |- | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf |- | Example || Example || Ex...")
- 19:30, 9 January 2018 (diff | hist) . . (-11) . . Chromatin Immunoprecipitation (→Prior to starting this section:)
- 19:30, 9 January 2018 (diff | hist) . . (+3) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 19:28, 9 January 2018 (diff | hist) . . (+6) . . Chromatin Immunoprecipitation (→Reverse Crosslinks of Protein/DNA Complexes to Free DNA)
- 19:28, 9 January 2018 (diff | hist) . . (-1) . . Chromatin Immunoprecipitation (→Prior to starting this section:)
- 19:27, 9 January 2018 (diff | hist) . . (+16) . . Chromatin Immunoprecipitation (→Immunoprecipitation)
- 19:24, 9 January 2018 (diff | hist) . . (+6) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 19:23, 9 January 2018 (diff | hist) . . (+19) . . Chromatin Immunoprecipitation (→Crosslinking, Lysis and Shearing of DNA)
- 19:19, 9 January 2018 (diff | hist) . . (+58) . . Chromatin Immunoprecipitation (→Immunoprecipitation)
- 19:17, 9 January 2018 (diff | hist) . . (-4) . . Chromatin Immunoprecipitation
- 19:14, 9 January 2018 (diff | hist) . . (-32) . . Chromatin Immunoprecipitation (→Couple the primary antibody for each transcription factor or chromatin protein to magnetic beads)
- 19:11, 9 January 2018 (diff | hist) . . (+3,234) . . Chromatin Immunoprecipitation (→Immunoprecipitation)
- 21:43, 5 January 2018 (diff | hist) . . (+2) . . Preparation of Protein Lysates from Cells (current)
- 18:33, 15 December 2017 (diff | hist) . . (-161) . . Preparation of Protein Lysates from Cells
- 18:28, 15 December 2017 (diff | hist) . . (+1,041) . . N Preparation of Protein Lysates from Cells (Created page with "==Materials== *RIPA Buffer (see RIPA) or other Lysis buffer. Add protease inhibitors. *Cells (fresh or frozen) ==Protocol== #Cool centrifuge to 4C #If cells...")
- 16:20, 24 October 2017 (diff | hist) . . (0) . . Submitting Plasmids for Sequencing (current)
- 16:19, 24 October 2017 (diff | hist) . . (+159) . . Submitting Plasmids for Sequencing
- 19:41, 19 October 2017 (diff | hist) . . (+114) . . Cloning CRISPR-Cas Plasmids (current)
- 19:38, 19 October 2017 (diff | hist) . . (+32) . . Submitting Plasmids for Sequencing
- 19:35, 19 October 2017 (diff | hist) . . (+752) . . Submitting Plasmids for Sequencing
- 19:26, 19 October 2017 (diff | hist) . . (+975) . . N Submitting Plasmids for Sequencing (Created page with "*Plasmids need to be diluted to a concentration of ~50ng/ul and total volume should be 10ul *These should be submitted in 1.5mL tubes *Go to https://client-seqcore.brcf.med.um...")
- 15:52, 11 August 2017 (diff | hist) . . (-8) . . Western Blotting (→Protocol)
- 15:52, 8 August 2017 (diff | hist) . . (+83) . . Bradford Assay (→Protocol)
- 15:51, 8 August 2017 (diff | hist) . . (-4) . . Bradford Assay (→Protocol)
(newest | oldest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)
